The eight regional centers, or AHECs, affiliated with the University of Kentucky are strategically located throughout the state:
- North Central AHEC – Florence, KY
- Northeast AHEC – Morehead, KY
- Southeast AHEC – Hazard, KY
- Southern AHEC – Mt. Vernon, KY
The AHECs affiliated with the University of Louisville are:
- Northwest AHEC – West Louisville, KY
- Purchase AHEC – Murray, KY
- South Central AHEC – Bowling Green, KY
- West AHEC – Madisonville, KY